Whole-genome expression analysis in the third instar larval midgut of Drosophila melanogaster

Journal article

Whole-genome expression analysis in the third instar larval midgut of Drosophila melanogaster

G3 (Bethesda, Md.) | GENETICS SOCIETY AMERICA | Published : 2014

Abstract

Copyright © 2014 Harrop et al. Survival of insects on a substrate containing toxic substances such as plant secondary metabolites or insecticides is dependent on the metabolism or excretion of those xenobiotics. The primary sites of xenobiotic metabolism are the midgut, Malpighian tubules, and fat body. In general, gene expression in these organs is reported for the entire tissue by online databases, but several studies have shown that gene expression within the midgut is compartmentalized. Here, RNA sequencing is used to investigate whole-genome expression in subsections of third instar larval midguts of Drosophila melanogaster. The data support functional diversification in subsections of ..

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University of Melbourne Researchers

Thomas Harrop Author

Philip Batterham Author

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Funding Acknowledgements

We thank Rob Good for bioinformatics support, discussion, and feedback on the manuscript and Emily Remnant for helpful comments on the manuscript. Funding support for this research was provided through an Australian Research Council Grant (DP1095295) to P. B. and P.J.D.